PAPER CHROMATOGRAPHIC SEPARATION OF SOME: BIOLOGICALLY IMPORTANT PHOSPHATE ESTERS

New alkyl-phosphate bonded stationary phases for liquid chromatographic separation of biologically active compounds

A new type of bonded stationary phase for liquid chromatography, with the properties of immobilized artificial membranes, has been synthesized. Alkyl-phosphate adsorbents were obtained by modification of aminopropyl silica gel. The structures of the synthesized materials were confirmed by use of instrumental techniques--elemental analysis, infrared spectroscopy (FTIR), and (13)C and (29)Si CP/M...

The chromatographic separation of uroporphyrin I and III octamethyl esters.

Improved Chromatographic Separation of Sitagliptin Phosphate and Metformin Hydrochloride.

New UPLC method was developed for determination of sitagliptin and metformin using Symmetry C18 column (100 mm × 2.1 mm, 2.2 μm) and isocratic elution (methanol 20%), pH (3.5) as a mobile phase. The ultraviolet detector was operated at 220 nm and the column temperature was 50°C. Linearity parameters were acceptable over the concentration ranges of 2-12 μgml(-1) and 5-35 μgml(-1) for sitagliptin...

The separation of esters of choline by filter-paper chromatography.

7. It is concluded that tran8-tran8-muconic acid is probably a true metabolite of benzene and is not produced in vivo from the ci8-ci8-acid. It is suggested that the 68-c68-acid need not be an intermediate in the formation of the tran8-tranm-acid in the animal body. The expense of this work was in part defrayed by a grant from the Medical Research Council. We are grateful to Prof. R. Cruickshan...

Separation of uroporphyrin esters I and III by paper chromatography.

Adams, M., Richtmyer, N. K. & Hudson, C. S. (1943). J. Amer. chem. Soc. 65, 1369. Bacon, J. S. D. (1952). Biochem. J. 50, xviii. Bacon, J. S. D. & Edelman, J. (1951). Biochem. J. 48, 114. Bacon, J. S. D. & Loxley, R. (1952). Biochem. J. 51, 208. Bawden, F. C. & Pirie, N. W. (1942). Brit. J. exp. Path. 23, 314. Bealing, F. J. & Bacon, J. S. D. (1951). Biochem. J. 49, lxxv. Bealing, F. J. & Bacon...